Journal: Frontiers in Cellular and Infection Microbiology

Article Title: CD8 + T cells promote tubule-interstitial damage in malaria-induced acute kidney injury

doi: 10.3389/fcimb.2025.1561806

Figure Lengend Snippet: Adoptive transfer of malaria-responsive T cells induces an increase in CD8 + T cells population in renal tissue. (A) Dot-plot representing the percentage of CD4 and CD8 + T cells in total CD45 + renal cells of the naive → naive (orange) and infected → naive (blue) groups. (B) Quantification of the percentage of CD4 + (n = 5) and (C) CD8 + T cells (n = 5) in the kidney. (D) Representative immunoblotting of cortical perforin-1 expression in renal tissue. (E) Quantification of perforin-1 expression in renal tissue (n = 4). *versus naive → naive group, P < 0.05.

Article Snippet: The cells were incubated with the following antibodies for surface markers: PeCy5.5-conjugated rat IgG2a anti-murine CD8 (45-0081-80, Thermo Fisher Scientific, Waltham, MA), PE-conjugated rat IgG2b anti-mouse CD4 (clone GK1.5; 11-0041-82, Thermo Fisher Scientific), and FITC-conjugated rat IgG1 anti-mouse CD45 (11.0452-82, Thermo Fisher Scientific).

Techniques: Adoptive Transfer Assay, Infection, Western Blot, Expressing

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: CD8 + T cells promote tubule-interstitial damage in malaria-induced acute kidney injury

doi: 10.3389/fcimb.2025.1561806

Figure Lengend Snippet: Depletion of CD8 + T cells induces an increase in parasitemia but does not change the percentage of CD4 T cells. (A) Representative scheme of CD8 + T cell depletion. Animals were infected with 10 6 iRBCs and treated with anti-mouse CD8 antibody on the day 2 and 3 post infection. The analyses were performed on day 5. (B) Peripheral parasitemia was assessed daily (n = 4). (C) Histogram representing the percentage of CD4 + and CD8 + T cells in CD45 + cells of the spleen. (D) Quantification of the percentage of CD4 + and CD8 + T cells in spleen (n = 4). (E) Histogram representing the percentage of CD4 + and CD8 + T cells in CD45 + cells of the kidney. (F) Quantification of the percentage of CD4 + and CD8 + T cells in spleen (n = 4). *versus undepleted group, P < 0.05.

Article Snippet: The cells were incubated with the following antibodies for surface markers: PeCy5.5-conjugated rat IgG2a anti-murine CD8 (45-0081-80, Thermo Fisher Scientific, Waltham, MA), PE-conjugated rat IgG2b anti-mouse CD4 (clone GK1.5; 11-0041-82, Thermo Fisher Scientific), and FITC-conjugated rat IgG1 anti-mouse CD45 (11.0452-82, Thermo Fisher Scientific).

Techniques: Infection